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optiprep density gradient medium  (Thermo Fisher)


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    Thermo Fisher optiprep density gradient medium
    Optiprep Density Gradient Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/optiprep density gradient medium/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    optiprep density gradient medium - by Bioz Stars, 2026-04
    99/100 stars

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    Assessment of the magnetic properties displayed by the crude cell extracts (CCEs) containing different citrine-ferritin fusion proteins using permanent neodymium ring magnets. The magnets were arranged in a 2×2 grid as shown in the leftmost panel, and covered with a black paper to aid visualization, onto which the mini petri dishes containing the CCE-OptiPrep density gradient medium mixtures (17% OptiPrep) were placed. Upon placement over the permanent magnets, the CCEs were left undisturbed for up to 69 h to follow the pattern formation. The contrast of all images was increased by 20%.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Magnetic protein aggregates generated by supramolecular assembly of ferritin cages - a modular strategy for the immobilization of enzymes

    doi: 10.3389/fbioe.2024.1478198

    Figure Lengend Snippet: Assessment of the magnetic properties displayed by the crude cell extracts (CCEs) containing different citrine-ferritin fusion proteins using permanent neodymium ring magnets. The magnets were arranged in a 2×2 grid as shown in the leftmost panel, and covered with a black paper to aid visualization, onto which the mini petri dishes containing the CCE-OptiPrep density gradient medium mixtures (17% OptiPrep) were placed. Upon placement over the permanent magnets, the CCEs were left undisturbed for up to 69 h to follow the pattern formation. The contrast of all images was increased by 20%.

    Article Snippet: To visualize the magnetic properties of citrine-ferritin fusions, 5 mL of crude cell extracts (CCE) of constructs overproducing the citrine-ferritin fusions were mixed with 1 mL of OptiPrep Density Gradient Medium (STEMCELL Technologies Germany GmbH, Köln, Germany) and transferred to mini petri dishes, corresponding to 10% iodixanol (w/v) concentration in the mixture.

    Techniques: